𝗠𝗲𝘁𝗵𝗼𝗱𝘀 𝗢ᶠ 𝗟ᵃᵇ 𝗗ᵃⁱᵍⁿᵒˢⁱˢ

 🔵 𝗩𝗘𝗧𝗘𝗥𝗜𝗡𝗔𝗥𝗬 𝗦𝗣𝗘𝗖𝗜𝗠𝗘𝗡 𝗙𝗢𝗥 𝗟𝗔𝗕𝗢𝗥𝗔𝗧𝗢𝗥𝗬 𝗗𝗔𝗜𝗚𝗡𝗢𝗦𝗜𝗦 :•✍︎

▪️Tissue samples are collected from dead or live animal for laboratory examination to confirm if there is adisease or not..

⚫𝗼𝗯𝗷𝗲𝗰𝘁𝗶𝘃𝗲 𝗼𝗿 𝗽𝘂𝗿𝗽𝗼𝘀𝗲 

✓Diagnosis of the disease.

✓Confirmation of tentative diagnosis

✓Prognosis

✓To observe the effect of treatment and give direction for future therapy.

ℹ️𝗚𝗘𝗡𝗘𝗥𝗔𝗟 𝗖𝗢𝗡𝗦𝗜𝗗𝗘𝗥𝗔𝗧𝗜𝗢𝗡𝗦 𝗙𝗢𝗥 𝗖𝗢𝗟𝗟𝗘𝗖𝗧𝗜𝗢𝗡 𝗢𝗙 𝗦𝗣𝗘𝗖𝗜𝗠𝗘𝗡𝗦: 

▪️➪ The diseases most commonly encountered in animals are of bacterial, viral, parasitic, fungal and metabolic origin. Diagnosis based on symptoms and laboratory examination of the relevant materials is essential for initiating treatment at the proper time. In general the following points should be duly considered while collecting materials for laboratory diagnosis.

▪️➪ All materials collected should be accompanied by full history of disease outbreak namely species affected, duration of disease, clinical signs, morbidity and mortality rates, disease suspected etc.

▪️➪ The collected biological specimens should be transported on ice to the nearest laboratory as early as possible.

▪️➪ Materials collected for bacteriological examination should be kept at refrigeration temperature (4 °C) in case of delay of transportation. If a viral etiology is suspected the material can be stored at –20°C to -80 °C.

▪️➪ When sero-diagnosis is required, collect paired serum samples (about 2 ml sera). One serum sample should be collected at the onset of disease and second sera after recovery (3-4 weeks) from disease preferably on 21st day.

▪️➪ If death is reported, the post-mortem examination should be conducted at the earliest as putrefied materials are unfit for laboratory examination & detailed post-mortem report should be attached along with the samples collected during post-mortem.

▪️➪ For Histopathological studies, tissues should be preserved in 10% formalin. The volume of formalin used should be approximately 10 times the volume of material. Specimen bottles with wide mouth should be used for collecting tissues.

▪️➪ All the impression smears before packing, should be fixed in methanol for 1-5 minutes unless otherwise specified.

▪️➪  In case of outbreaks, try to collect materials from as many ailing animals (5-6 or more) as possible at the height of body temperature /clinical signs.   

                                             

𝘄𝗿𝗶𝘁𝘁𝗲𝗻 𝗯𝘆: 𝗦ᵒᵐᵃˡⁱ 𝗩ᵉᵗᵗᵉʳⁱⁿᵃʳʸ 𝗗ᵒᶜᵗᵒʳˢ✍︎✍︎